ENRegional development is a complex process that can be analysed in various contexts, including environmental, social and economic factors. Variations in the levels of development are naturally observed across countries and regions, but they play a special role in trans-border regions. The aim of this study is to investigate diferences in the level of development of two Polish voivodships (Warmia and Mazury, and Podlasie) and two Lithuanian counties (Marijampolė and Alytus) which are trans-border regions (NUTS 4 level). The study was conducted by analysing three subordinate criteria, namely environmental, social and economic factors, as well as the overall development of the evaluated regions with the use of the analytical hierarchy process and technique for order preference by similarity to ideal solution methods. The evaluated trans-border regions are characterised by a predominance of rural areas and considerable variations in development. The entire area is characterised by high natural value. This is an important observation because the condition of the natural environment is a key determinant of well-being, and it directly infuences the quality of life. The analysis revealed considerable demographic problems, mainly in Lithuanian regions, resulting from low population growth and a negative net migration rate which infuence population structure. Disproportions in regional development call for regional policies that are adapted to local needs and resources. Keywords: Regional development, Trans-border regions, Polish and Lithuanian regions, TOPSIS, AHP, Synthetic indicator. [From the publication]